Glaucoma is a disease characterized by damaging the optic nerve head, this can result in severe vision loss. An early detection and a good treatment provided by the ophthalmologist are the keys to preventing optic nerve damage and vision loss from glaucoma. Its screening is based on the manual optic cup and disc segmentation to measure the vertical cup to disc ratio (CDR). However, obtaining the regions of interest by the expert ophthalmologist can be difficult and is often a tedious task. In most cases, the unlabeled images are more numerous than the labeled ones.We propose an automatic glaucoma screening approach named Super Pixels for Semi-Supervised Segmentation “SP3S”, which is a semi-supervised superpixel-by-superpixel classification method, consisting of three main steps. The first step has to prepare the labeled and unlabeled data, applying the superpixel method and bringing in an expert for the labeling of superpixels. In the second step, We incorporate prior knowledge of the optic cup and disc by including color and spatial information. In the final step, semi-supervised learning by the Co-forest classifier is trained only with a few number of labeled superpixels and a large number of unlabeled superpixels to generate a robust classifier. For the estimation of the optic cup and disc regions, the active geometric shape model is used to smooth the disc and cup boundary for the calculation of the CDR. The obtained results for glaucoma detection, via an automatic cup and disc segmentation, established a potential solution for glaucoma screening. The SP3S performance shows quantitatively and qualitatively similar correspondence with the expert segmentation, providing an interesting tool for semi-automatic recognition of the optic cup and disc in order to achieve a medical progress of glaucoma disease.